Herbal medicines are those with active ingredients made from plant parts, such as leaves, roots or flowers. Boswellia serrata (Salai/Salai guggul), is a moderate to large sized branching tree of family Burseraceae (Genus Boswellia), grows in dry mountainous regions of India, Northern Africa and Middle East. This study investigates the qualitative, quantitative and in vitro antimicrobial activity of Boswellia serrata plant extract. The roots of Boswellia serrata was collected and was treated by maceration process using Hydroalcoholic solvent (methanol: water 80:20). The percentage yield of Hydroalcoholic extract of Boswellia serrata was found to 5.12% by using maceration method. The results of phytochemical revels that the all polar and aqueous soluble compound was found to be present in Boswellia serrata roots extract. The total alkaloid content was found to be 0.215 AT mg/100mg. The results of antimicrobial study shows that the aqueous extract of roots of Boswellia serrata have promising antibacterial effect. The results of antimicrobial activity showed that the plant extract is effective against bacteria as well as fungi. Hence it is concluded that the roots of Boswellia serrata can be used in pharmaceutical industry for the formulation of antimicrobial drug for the treatment of various fungal and bacterial infections. Key Words: Boswellia serrata, Antimicrobial Activity, Phytochemical analysis, Pharmaceutics