Psidium guajava L, belonging to the Myrtacea family, has been reported to have anti-diarrheal, hepatoprotective, hypoglycemic, lipid lowering, antibacterial and antioxidant activities. It contains important phytoconstituents such as tannins, triterpenes, flavonoid: quercetin, pentacyclic triterpenoid: guajanoic acid, saponins, carotenoids, lectins, leucocyanidin, ellagic acid, amritoside, beta-sitosterol, uvaol, oleanolic acid and ursolic acid. Thin layer chromatography has become widely used method for the separation of substances in a great variety of inorganic and organic studies. This investigation aimed to perform the extraction, phytochemical screening and TLC of Psidium guajava. The results showed that the yields were found to be (7.39% w/w of crude drug) of Methanolic extract Psidium guajava leaves. Obtained results were recorded in table 1. Results of Phytochemical test showed the presence of Carbohydrates, Flavonoids, Proteins & Amino acids, Diterpenes, Saponins, Alkaloid and phenols (Table 2). Total Flavonoid content was 0.165mg/100mg quercetin equivalent in extract. The TLC was also performed to identify the presence of favanoids.It shows that the presence of such bioactive compounds has medicinal importance. Hence Psidium guajava was found to have medicinal properties. Key words: Herbal Medicine, Psidium guajava, Thin Layer Chromatography, Flavonoid