The idea that natural remedies are safer and have fewer side effects than synthetic ones makes them more acceptable. The demand for herbal formulations is rising on the global market. Matricaria chamomilla (synonym: Matricaria recutita), commonly known as chamomile (also spelled camomile), is an annual plant of the composite family Asteraceae. Chamomile is known mostly for its use against gastrointestinal problems; additionally, it can be used to treat irritation of the skin. The purpose of this study is to test the herbal gel of Matricaria chamomilla for effective treatment of topical microbes. The flowers of plant Matricaria chamomilla were collected and subjected to extraction aby Maceration. The extractive value was determined in terms of percentage yield & phytochemical analysis was also performed. The prepared gel was tested for various parameters along with antimicrobial activity. The results showed that the percent yield was found to be 7.21%. Phytochemical test showed the presence of various bioactive compounds such as Carbohydrates, Flavonoids, Proteins & Amino acids, Diterpenes and Saponins. The total flavonoid content was found to be 0.832mg/100mg. The herbal gel evaluation showed that all of them have clear colour, No clogging, good homogeneity and smooth texture. In all formulations of gel the spreadibility and viscocity of HG5 is good was found to be 12.12±0.15 and 3650±25. The skin irritation test performed showed no signs of sensitivity, erythema and edema. The antimicrobial study revealed that the formed gel have zone of inhibition of 12±0.5 against P. acne at 100 mg/ml concentration. So the prepared formulations were considered to be non-irritant with appreciable antimicrobial activity. Keywords: Matricaria chamomilla, Herbal gel, Topical microbes, anti-microbial activity