The published files are two maps of North American boreal forest tree cover trends between 2000 and 2019. Pixel values are annual trends in tree cover expressed as % change per year. The trends are based on annual tree cover estimates from the MODIS Vegetation Continuous Field version 6 product. We quantified tree cover trends per pixel through Theil-Sen's slope estimation using the 'zyp' package in R. We followed the Yue-Pilon pre-whitening method to account for temporal autocorrelation. We created a trend map for all data points within the boreal biome boundary following Gauthier et al. 2015, Science (DOI: 10.1126/science.aaa9092) and added a 120km buffer around it (tcchange_all_points_clipped.tif). We also produced a map where we masked out non-significant trends based on a Mann-Kendall-test (tcchange_significant_points.tif). Both maps have a spatial resolution of around 1,000m. The map forms the key results in our manuscript: Rotbarth et al. 2023. 'North American boreal forests: Northern expansion is not compensating for southern declines'. Nature Communications