This repository contains source data and processed data accompanied with the paper: "Distinct mobility patterns of BRCA2 molecules at DNA damage sites" The repository contains the following files: - Movies: This folder contains the movies that were used for 3D single-molecule tracking. As described in the methods section of the paper, each movie consists of a 3D stack of 9 image planes that were registered using fluorescence beads from the original data. - Trajectories: These CSV files contain the results of 3D tracking using the Mosaic tracking plugin in ImageJ. Traj_* contains the tracking results where the column inMask column indicates whether a localization is inside the DNA damage mask. Traj_transformed_[1-3]_* contains the corresponding tracking data in which the mask was transformed, which was used as a control in the paper. - segment files: The data that was used in the subsequent R scripts. - segs_nest.Rdata is the same data as the segment files but in Rdata file format, to be imported in R For questions please contact Maarten Paul (m.w.paul [at] erasmusmc.nl)