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FORMULATION, DEVELOPMENT AND MICROBIOLOGICAL STUDY OF APHRODISIAC CAPSULE, AND IDENTIFICATION AND DETERMINATION OF GALLIC ACID IN EMBELICA OFFICINALE BY HPTLC

Authors: Yogesh B. Bawne, Sharda L. Deore;

FORMULATION, DEVELOPMENT AND MICROBIOLOGICAL STUDY OF APHRODISIAC CAPSULE, AND IDENTIFICATION AND DETERMINATION OF GALLIC ACID IN EMBELICA OFFICINALE BY HPTLC

Abstract

Research has been proposed. The polyherbal formula, only herbal Extract is taken to developed to efficient sexual activity and increase the sexual intercourse, Hydrolysable tannins convert into Gallic acid, Induce erectile dysfunction and antioxidant activity; it is more efficient with Sildenafil and also with Aphrodisiac natural compound. A polyherbal formula was developed which has mostly aphrodisiac activity and antioxidant activity. Raw materials evaluation and specifications were confirmed before development. The specified tannin assay of Awala extract was found to be 27.08% and the total tannins in the Aphrodisiac herbal capsule was found to be 8.31%. 0.67% and 0.93% Gallic acid was calculated in Awala extract and finished capsule. The percentage of saponin is calculated in the finished capsule at about 16.23%. For standardization and identification of gallic acid, the standard Gallic acid was ordered from Sigma Aldrich Company which was 97% pure. The chromatograph of Gallic Acid from Extract, Chromatogram of Gallic acid from Capsule and Chromatogram of Standard Gallic acid were an exact match in the overlaying spectrum of chromatographic conditions. Stability study of aphrodisiac product maintained at Accelerated Conditions (: 40 0c ± 2 0c/ 75 Rh ± 5 0c). Materials & Instruments: Camag HPTLC system (Anchrom, Switzerland) consisting of Camag Linomat-V, Camag twin trough Chamber with stainless steel lids for chromatographic development, Camag TLC Scanner- III equipped with cats 4 software for scanning and documentation, and Camag UV cabinet. UV Spectrophotometer. PH meter, Toshcon instruments Pvt Ltd Haridwar. Horizontal laminar Air Flow, kirloskar India Ltd Mumbai. Electronic weighing balance, Mettler Toledo, AG 135, Tokyo. Muffle furnace, Tempo Instruments and Equipments (p) Ltd, Mumbai. Sonicator, Bieora Enterprises, Mumbai. Incubator, Elite Scientific instruments company, Bombay. Chemicals and Media: All chemicals were of analytical grade or HPLC grade. The raw extracts were obtained from Phyto Life Sciences P. Ltd. Ahmadabad, Gujarat, India. Markers were used for analytical method development and quantitative analysis. They were purchased from supplier Sigma Life Sciences Pvt, Ltd. by Siddhayu Ayurvedic Research Foundation Pvt. Ltd. Bahadura. Nagpur. Conclusion: As per results and Observation, we concluded that all polyherbal formulations should contain natural awala or awala extract. And doctors may write additional vitamin c tablets along with other medication to increase the effectiveness of their medication. Overlining the spectra of three chromatograms was the same at every point, so we concluded and confirmed that standardization of Gallic acid has been successful. During the stability study, the parameters and specifications of aphrodisiac capsules were not changed. Those changed but they were within limits, and that all the activities and efficacy of natural organic compounds were still active. Keywords: Gallic acid, Ellagic acid, Embillica officinale, Goose barry, Awala, Antioxidant, sexual Dysfunction, HPTLC

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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