The mental and psychological consequences of Burnout affect the health-related quality of care. This study aimed to assess Burnout level among the nurses in Najran region in the Kingdom of Saudi Arabia. A descriptive cross-sectional design was used. The sample of the study was a census consisted of 161nurses. The researcher used a self-administered questionnaire, adopted the international Maslach Burnout Inventory-Human Service Survey third edition to study the Burnout for data collection. For data analysis, the researcher used the SPSS program (version 22). Statistical analysis included frequency, mean, and percentage, as well as independent sample t-test and one-way ANOVA. Results: Most participants were females (66.5%) and aged 30 - 40 years (47.2%). about (44.7%) had high EE, (32.3%) had high DP and (40.4%) had low PA. In general, the overall mean score of all the subscales was (2.928), with a mean percent of (48.80%), which reflected a low level of Burnout among study participants. Burnout was associated with age, and working overtime. Conclusion: Level of burnout among nurses was low and was associated mainly with stressors in the workplace. Improving ergonomics and managing stress in the workplace should be a priority to reduce burnout among nurses. Keywords: Governmental hospitals, burnout, nursing, Saudi Arabia