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ZENODO
Other literature type . 2022
License: CC BY
Data sources: ZENODO
ZENODO
Other literature type . 2022
License: CC BY
Data sources: Datacite
ZENODO
Other literature type . 2022
License: CC BY
Data sources: Datacite
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Purification of SLC12A5 from Expi293FTM Cells

descriptionPublicationkeyboard_double_arrow_right Other literature type 21 Dec 2022 English Publisher:ZenodoFunded by:EC | ReSOLUTE
Authors: David B. Sauer; Huanyu Li; Sagar Raturi; Andreea Scacioc; Tina Bohstedt; Abilasha Balakrishnan;

doi: 10.5281/zenodo.7468696 , 10.5281/zenodo.7468697

Purification of SLC12A5 from Expi293FTM Cells

Abstract

This protocol is used to purify the SLC transporter expressed in Expi293FTM Cells using detergent solubilization followed by a series of different chromatographic methods. Depending on the nature of the expression construct and experimental requirements, the GFP tag can be removed using a proteolytic reaction.

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    popularity
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    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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