The folder related to the paper " Glycoproteomics of a single protein: revealing tens of thousands of Myozyme® glycoforms by hybrid MS approaches" by Di Marco et al. contains the following 9 raw files, 3 excel files and 4 Byonic files (2 byrslt and 2 byspec2): Raw files: PGC-HPLC-MS/MS analysis of Myozyme® released N-glycans (File name: PGC_HPLC_MS_MS Released N_glycans Myozyme.mzML) Triplicates of nano RP-HPLC-MS/MS analyses of Myozyme® glycopeptides (File name: nano RP_HPLC_MS_MS Myozyme glycopeptide 1, 2 and 3.raw) micro RP-HPLC-MS/MS analyses of Myozyme® deglycosylated peptides by PNGase F (File name: micro RP_HPLC_MS_MS Myozyme PNGase F peptide not alk cysteinylation.raw) RP-HPLC-MS analysis deglycosylated intact Myozyme® by PNGase F in non-reducing condition (File name: RP_HPLC_MS PNGase F Myozyme non-reduced.raw) RP-HPLC-MS analysis deglycosylated intact Myozyme® by PNGase F in reducing condition (File name: RP_HPLC_MS PNGase F Myozyme reduced.raw) SAX-HPLC-MS analysis of intact Myozyme® (File name: SAX_HPLC_MS intact Myozyme.raw) SAX-HPLC-MS analysis of desialylated intact Myozyme® by sialidase (File name: SAX_HPLC_MS sialidase intact Myozyme.raw) Excel files: Results of Byonic glycopeptide search (File name: Byonic search results Myozyme glycopeptides.xlsx) Results of Byonic PNGase F peptides, not alkylated, cysteinylated (File name: Byonic search results Myozyme PNGase F peptides not alk cysteinylation.xlsx Report of Skyline with the results of glycopeptide EICCs integration Byonic files: Results of Byonic glycopeptide search (File name: Byonic search results Myozyme glycopeptides.byrslt and Byonic search results Myozyme glycopeptides.byspec2) Results of Byonic PNGase F peptides, not alkylated, cysteinylated (File name: Byonic search results Myozyme PNGase F peptides not alk cysteinylation.byrslt and Byonic search results Myozyme PNGase F peptides not alk cysteinylation.byspec2)