Systemic sclerosis (SSc) is a chronic and incurable autoimmune disease with high mortality rates, and fibrosis is the distinguishing hallmark in the pathogenesis. Identification of the precise, time dependent composition of stromal and immune cells may provide clues for the establishment of new biomarkers and therapeutic approaches targeting SSc fibrosis. Here, the temporal dynamics of pathological process in a mouse model of skin fibrosis is investigated using single-cell RNA-sequencing (scRNA-seq). we collected skin single cell suspensions for transcriptome profiling by scRNA-seq from four time points after BLM-treatment (day 3, 7, 14, and 28) and untreated control skin (day 0) with on average three replicate mice per time point, and repeated once at each time point.{mouse_SSc_scRNAseq_day0_sample1_B1_1, mouse_SSc_scRNAseq_day3_sample1_B2_1, mouse_SSc_scRNAseq_day3_sample2_B2_2, mouse_SSc_scRNAseq_day7_sample1_B3_1, mouse_SSc_scRNAseq_day7_sample2_B3_2, mouse_SSc_scRNAseq_day14_sample1_B4_1, mouse_SSc_scRNAseq_day14_sample2_B4_2, mouse_SSc_scRNAseq_day28_sample1_B5_1, mouse_SSc_scRNAseq_day28_sample2_B5_2}