The article was dedicated to investigation of polymorphism of hordrein reserve proteins. Electrophoretic spectra of 32 samples of local and introduced barley varieties were analysed. The genetic proximity of genotypes was assigned by SPSS computer method, cluster analysis was applied to results and then barley samples were devided into 6 groups based on the Jaccard similarity index. Frequencies of occurrence of electrophoretic patterns and spectra were identified based on zones оf samples. The genetic diversity indexes for each zone have been counted according to the Nei formula.