Good quality DNA is the prerequisite for any type of biotechnological research. Several protocols are described by many researchers as well as quite a few dozen commercial DNA isolation kits are available in the market. Most of the protocols are very specific for typical taxonomic group and may not have similar quality of DNA with different taxa. Under this study we have developed a protocol which is universal for all plant taxa and can be used in isolation of DNA from live, dried and alcohol preserved tissues of variety of plants taxa. This protocol is relatively economical as it does not involve the RNAse-A and Proteinase-K use. It is environmental friendly as no use of phenol and non-hazardous since no use of liquid nitrogen. The protocol is proved to be less time consuming with better DNA yield from small tissue