Within the frame of the ELECTRA No. 826244 H2020 project, a sequential reductive/oxidative bioelectrochemical process at a laboratory scale was tested with real groundwater from a contaminated site in Northern Italy for chlorinated aliphatic hydrocarbons (CAHs) removal. The changes in groundwater ecotoxicity were followed by a complex ecotoxicity test battery including testorganisms from various trophic levels. The datasets (supplementary to Chapter 3.5) for this publication are presented here. Referred publication: Edoardo Dell'Armi, Marco Zeppilli, Maria Letizia Di Franca, Bruna Matturro, Viktória Feigl, Mónika Molnár, Zsófia Berkl, Imre Németh, Hafsa Yaqoubi, Simona Rossetti, Marco Petrangeli Papini, Mauro Majone, Evaluation of a bioelectrochemical reductive/oxidative sequential process for chlorinated aliphatic hydrocarbons (CAHs) removal from a real contaminated groundwater, Journal of Water Process Engineering, 49, 2022, 103101, https://doi.org/10.1016/j.jwpe.2022.103101.