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Results of this study show that trehalose, caffeine and glutathione supplementations have stimulating effects on spermatozoa motility parameters. Stimulating effects of trehalose were lasting over a longer time period, with recommendation of trehalose supplementation for a long-time spermatozoa cultivation. Caffeine and glutathione have the most markedly stimulating effects especially over the first 24 hours of cultivation, and they could be used as additives for a short-term in vitro spermatozoa preservation.
motility, spermatozoa, glutathione, trehalose, bulls, caffeine
motility, spermatozoa, glutathione, trehalose, bulls, caffeine
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