Cancer is one of the deadliest diseases, and its early detection and timely treatment are extremely important. The use of 3D cultures (spheroids) is the most relevant approach in cancer research because it mimics the in vivo environment of cells. Monitoring the tumor marker expression with different diagnostic methods is very important in determining specific therapy. Loop-mediated isothermal amplification (LAMP) is an innovative molecular tool that has a wide application, including the field of cancer diagnostics.