Pseudomonas aeruginosa is the main pathogen causing nosocomial infections in hospitals, also can infect open wounds, combustio and necrotizing pneumonia. Several plant studies show the presence of antioxidants, one of them is red ginger. Research shows that red ginger extract contains the bioactive components gingerol and shogaol which have antioxidant and antimicrobial properties. The purpose of this research is to determine the effect of red ginger extract on TNF-α and serum malondialdehyde levels in rats infected with Pseudomonas aeruginosa bacteria. This study uses an experimental research design with Post Test Only Control Group Design. The research subjects were 30 wistar rats which were randomly divided into 5 groups. K (-) group of mice without P.aeruginosa infection. K(+) group of rats infected with P. aeruginosa 108 CFU per head without red ginger extract. P1, P2 and P3 were infected with P.aeruginosa and were given red ginger extract at a dose of 20mg, 40mg and 80mg/KgBB/day for 7 days. Day 8 examination of TNF-α and MDA levels. The results of the One Way Anova test showed a significant difference in TNF- levels and MDA levels with p value = 0.000. The mean levels of TNF- in groups K(-), K(+), P1, P2, P3 were 5.76, 17.89, 12.29, 9.03, 7.07 and the mean MDA levels were 1.33, 11.10, 5.09, 3.87, 3.00. Tukey's test results showed TNF-α levels between groups had a significant difference in all groups (p=0.000) and a significant difference also in MDA levels (P=0.000). Red ginger extract had an effect on decreasing TNF-α and serum MDA levels in rats infected with Pseudomonas aeruginosa.