Dataset 1: SH-SY5Y cells recorded on a confocal brightfield microscope. Speed: Image frequency: 1 seconds per image. Microscope: Leica SP8, 63x 1.40 NA oil objective Image size: 512 x 512 x (time or z dimension varying depending on the depth of the stack or the duration of the recording) File format: .tif (8-bit) Cell Type: SH-SY5Y Comments: Consisting of a mix of axial stacks and temporal sequences. Dataset 2: Fluorescently labelled lysosomes in SHSY5Y cells recorded on point-scanning confocal microscope. Speed: Image frequency: 1 seconds per image. Microscope: Leica SP8, 63x 1.40 NA oil objective Image size: 512 x 512 x (time or z dimension varying depending on the depth of the stack or the duration of the recording) File format: .tif (8-bit) Cell Type: SH-SY5Y, labelled with FLCS1 (fluorescent Copper(I) ligand which accumulates in lysosomes: see https://doi.org/10.1002/anie.202109349) Comments: Consisting of a mix of axial stacks and temporal sequences. Dataset 3: GFP labelled Dictyostelium discoideum cells recorded on inverted spinning disc confocal microscope. Speed: Image frequency: 2-minutes per image. (was originally recorded as 3D+T dataset - here reassembled as 2D+T) Microscope: inverted spinning disc con-focal microscope (3i) using 63x 1.4NA oil objective and equipped with a prime95B CMOS camera (photometrics). Image size: 512 x 512 (downsized from an original image size of 1200 x 1200 and augmented by zooming into the corners of the original image) File format: .tif (8-bit) Cell Type: Dictyostelium discoideum cells Dataset 4: Simulated particles created with ICY ISBI particle tracking challenge 2015 plugin Speed: - Microscope: - Particle and Image properties: Switching Uniform; xy pixel size: 15; y borders simu: 50; extinction rate: 0.003; density: 40; SNR: 10; sequence length: 225; Sigma brownian: 1; q1: 2; vmin: 2; vmax: 12; Probe directed Brownian: 0.3; Probe Brownian directed: 0.3 Image size: 512 x 512 File format: .tif (8-bit) Cell Type: -