This dataset contains a set of ground data and four flights captured on grape harvest over a vineyard affected by Botrytis cinerea. UAV flights took place on 16 September 2021, at 30 m height and using different angles (0, 30, 45 degrees). Pictures were taking using a Micasense RedEdge 3 sensor and were calibrated using the provided Micasense reflectance panel. The flight path was programmed to fly in autonomously, following manufacturer’s instructions (DJI). The dataset includes a shapefile with the GPS location of vine trunks, bunches affected by Botrytis and GCP points.