A simple, precision and accuracy HPLC method was developed the estimation of Remdesivir analysis of formulation, consisting of an Methanol: water (60: 40 % v/v). The chromatographic condition was set at a Flow rate of 1 ml/min with the UV detector at 240 nm. The above method was optimized with a view to develop an assay method for Remdesivir. Several mobile phase compositions were tried to resolve the peaks of Remdesivir. The optimum mobile phase containing methanol: water (60: 40 % v/v) was selected because it was found ideal to resolve the analyte peaks of the drug. Quantification was achieved with UV detections at 240 nm based on peak area and absorbence. As per USP requirements system suitability studies were carried out and freshly prepared standard solutions of Remdesivir. Keywords: Remdesivir, RP‐HPLC, Method development, Validation