In Institoris et al (2022), published in Nature Communications, we propose that a fundamental role of astrocyte Ca2+ is to amplify the late component of functional hyperemia when neuronal activation is prolonged. The code and example data provided here are intended to clarify the following statement in our manuscript: "Recordings of a small area of the Styrofoam ball were analyzed by a custom MATLAB script to quantify ball movement on a relative locomotion scale where ‘0’ is assigned to the least movement (stationary ball) and 1 is the highest speed an animal reached during the experiment."