The document contains all the primers identified for the screening of genes tested for their potential as biomarkers to predict quality performance in gilthead sea bream and European sea bass larvae and juveniles in the context of PERFORMFISH (WP2). The spreadsheet has the following information: Pathway, phisiologic process in which the gene is involved; name of protein that gene produces; gene code; acession nº, code given in the consulted databases and the sequence extracted for primer design; FW and RV primer, forward and reverse primer sequence specific for target gene; melt temperature, optimized temperature that primers work at ; amplicon size, size in base pairs of the product produced with the primers; eff%, efficency of primers; r2; source, the origin of the primers, "in house" or "literature" (including available DOI. Each pair of primers are classified using a "traffic light" system indicating their validation status.