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THE EFFECT OF TREHALOSE, CAFFEINE AND GLUTATHIONE ON BOVINE SPERMATOZOA: 2. MORPHOLOGY AND OXIDATIVE STATUS IN VITRO

descriptionPublicationkeyboard_double_arrow_right Article 15 Jun 2012Publisher:Zenodo

Authors: PETER MASSÁNYI; EVA TVRDÁ; MARTINA RAFAJOVÁ; NORBERT LUKÁČ;

doi: 10.5281/zenodo.7260814 , 10.5281/zenodo.7260813

THE EFFECT OF TREHALOSE, CAFFEINE AND GLUTATHIONE ON BOVINE SPERMATOZOA: 2. MORPHOLOGY AND OXIDATIVE STATUS IN VITRO

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Abstract

The aim of this study was to examine the effects of glutathione, trehalose and caffeine on selected bovine spermatozoa morphology parameters, as well as lipid peroxidation following an in vitro cultivation at different temperatures and time periods. Morphological analysis showed similar occurrence of morphological abnormalities in all group with no significant differences (p>0.05). The TBARS assay revealed that the selected concentrations of trehalose, caffeine and glutathione did not have a significant effect (p>0.05) on lipid peroxidation as a process leading to oxidative stress development in spermatozoa.

Related Organizations

Slovak University of Agriculture
Slovakia

Keywords

spermatozoa, MDA, morphology, glutathione, trehalose, bulls, caffeine

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