The target of this in vitro study was to analyse the influence of copper on the spermatozoa motility in the presence of bovine serum albumin (BSA) as culture medium and to provide additional information on the interaction between serum albumin and copper (II) chloride (CuCl2). The spermatozoa motility parameters was determined after exposure of CuCl2 (3.9; 7.8; 15.6; 31.2; 62.5; 125; 250; 500; 1000 μmol/L) using the Sperm VisionTM CASA (Computer Assisted Semen Analyzer) system during different time periods (Time 0 h, 1 h, 2 h and 24). The culture medium containing 20% BSA, triladyl and 5% glucose increased the overall percentage of spermatozoa motility after 1 h of cultivation. The percentage of motility spermatozoa significantly (P<0.001) decreased after 2 h of cultivation at the concentrations ≥ 250 μmol/L of CuCl2 in comparison with the control group (without CuCl2 administration). The experimental administration at the doses ≤ 31.2 μmol/L of CuCl2 stimulated the overall of motile spermatozoa (Time 24 h). Identical spermatozoa motility was detected also for the percentage of progressive motile spermatozoa during all time periods. Parameter of distance average path (DAP) showed increase in all CuCl2 addition groups in comparison with the control group at Time 1 h. Concentration 125 μmol/L of CuCl2 in various time periods of cultivation act stimulating on spermatozoa motility, but later (Time 24 h) inhibitory. Evaluation of velocity average path, showed similar results as for DAP. Measurement of the amplitude of lateral displacement (ALH) at Time 0 h as well as at Time 1 h was higher in all the experimental groups compared to the control group, but the differences were not significant (P>0.05). The experimental administration at the doses ≤ 62.5 μmol/L of CuCl2 stimulated ALH during 24 h of cultivation. The results suggest that adding energy and protein substrate to the culture medium increases the spermatozoa motility parameters also the presence of high doses (≥ 125 μmol/L) of copper ions during short-term periods (Time 0 h, 1 h). Concurrently BSA maintained motility of spermatozoa (≤ 31.20 μmol/L of CuCl2) during the long-term (Time 24 h) of cultivation, which confirms the protective effect of albumin binding to the copper ions.