ABSTRACT A four new cell lines viz., Bm-1, Bm-12 Bm-16, Bm-17 and Bm -5 is a worldwide used cell line are tested for BmNPV (Bombyx mori nuclepolyhdedrovirus) susceptibility and its replication The BmNPV was serially passaged in the Bm-12, Bm-1, Bm-16, Bm-17 and Bm-5 cell lines for five times. All the cell lines were found susceptible to BmNPV infection when inoculated with the haemolymph of BmNPV infected silkworm larvae. The infection rate was Bm-5 (93.79%), Bm-16 (91.30%), Bm-1 (90.30%), Bm-12 (87.20%) and Bm-17 (80.43%). Keywords: Bombyx mori, Ovarian cell lines, BmNPV infection REFERENCES Granados, R.R., McKenna, K.A., 1995. Insect Cell Culture Methods and Their Use in Virus Research: In: Schuler, M.L., Wood, H.A., Granados, R.R., Hammer, D.A., (Eds.), Baculovirus Expression Systems and Biopesticides. Wiley-Liss. New York, pp. 13-39. Khurad, A.M., Mahulikar, A., Rathod, M.K., Rai, M.M., Kanginakudru, S., Nagaraju, J., 2004. Vertical transmission of nuclear polyhedrosis virus in the silkworm, Bombyx mori L. J. Invertebr. Pathol.. 87, 8-15. Khurad, A.M., Mahulikar, A., Rathod, M.K., Rai, M.M., 2005. Infection of nuclear polyhedrovirus in the larval rudiments of gonads of silkworm, Bombyx mori L. Indian J. Seric. 44(2), 159-164. Khurad, A.M., et al., 2009. A new continuous cell line from larval ovaries of silkworm, Bombyx mori. In Vitro. Cell. Dev. Biol.- Animal. C. G. Deshmukh, R. S. Bahekar. “Comparative in vitro replication and serial passaging of BMNPV in the DZNU-Bm-12 and other cell lines” in International Journal IJSRP volume 3 ISSN No.2250-2153 Krishnaswami, S., M.N. Narasimhanna, S.K. Suryanarayan & S. Kumararaj, 1973. K. Srivastava and V.B. Upadhyay. 2013. Effect of phytoecdysteroid on fecundity of multivoltine mulberry silkworm Bombyx mori Linn. Biolife. 1(2): 78-83. T.V. Sathe, B. V. Jadhav, A.S. Desai, Nilam Shendge, Chandani Kamble and A. D. Jadhav. 2014. Ecology, ethology and control of green stink bug Plautia affinis Dallas (Hemiptera: Pentatomidae) on mulberry Morus alba l. Varieties V-1 and M-5 from Kolhapur. Biolife. 2(4); 1347-1353.