Background: Platelet-rich plasma (PRP) is blood plasma containing an above baseline level of platelets and growth factors. Recently, Platelet-rich plasma (PRP) has come to the attention of aesthetic surgeons, with burgeoning new applications ranging from hair restoration to soft tissue augmentation. Injectable Poly-D,L-Lactic Acid (PDLLA) is a biostimulator rather than a traditional filler, which provides immediate volumetric improvement. Injectable PLA can produce a result comparable with Hyaluronic Acid fillers for the correction of wrinkles such as nasolabial folds, and can be a good choice for such a treatment. Methods: A Female 45 years Old with deep smile lines and fine wrinkles on cheeks. The patient was treated with injected of Poly(D,L Lactic Acid) and Platelet Rich Plasma. Poly-D,L-Lactic Acid was injected subdermal with retrograde linear thread technique and used 24-G cannula needle. After 3 weeks PDLLA Injection, PRP was injected intradermal into the whole face especially along the smile lines using a 32-G Needle at a dose of 0.05 ml at each injection site with a distance of about 0.5-1.0 cm. PRP was injected every 2 weeks for 3 months. Results: Wrinkle Severity Rating Scale was downgrading and skin quality like skin tone looks more brightening and fading hyperpigmentation. Conclusion: The combination of Poly-D,L-Lactic Acid and Platelet-Rich Plasma is effective for facial rejuvenation. Keywords: Platelet-rich plasma (PRP), Poly-D,L-Lactic Acid, Nasolabial Folds, Wrinkle Severity Rating Scale