The present investigation is carried out using effluent water from the dyeing industries of Bhiwandi city. Most of the chemicals present in the effluent water when discharged into main water bodies consume oxygen for oxidation and decomposition purposes. They reduce the level of oxygen in water which in turn causes mortality of the bio-organisms. The dyeing industry creates water pollution as the waste generated from the different wet processes like scouring, bleaching, dyeing and printing are very harmful. They cause chemical pollution due to discharge of various chemicals and disturb the normal functioning of living organisms (bioorganisms) in water. This research work has been carried out to assess the effect of pollution on the quality of water (which could also be a source of drinking water) and on bio-organisms. Water samples collected from the dyeing industry were analyzed for amount of Oxygen and for biological parameter. It has been observed that reduction in dissolved oxygen concentration is one of the most important factors that directly affect the lifecycle of most of the aquatic animals. The results of oxygen consumed by the chemicals are then compared with the standard values prescribed by the standard bodies