In this study, we aimed to interpret the effect of using aerobic and anaerobic blood culture bottles together and the blood volume taken in the detection of circulatory system infections as soon as possible. Blood cultures were determined using BD BACTEC 9240 (Becton Dickinson, USA) as well as standard microbiological methods. The growth and growth times of isolates in aerobic and anaerobic blood culture bottles were compared and measured. 8178 out of a total of 11234 blood culture bottles were evaluated. Microbial growth was detected in 974 (11.9%) blood cultures. The main pathogens considered causative agents are coagulase-negative staphylococci 114 (18%), S. aureus 108 (17.1%), Klebsiella spp 86 (13.6%)., E. coli 63 (9.9%)., yeast 45 (7.1%)., and Acinetobacter spp 43 (6.8%) were detected. The clinically significant growth rate in blood cultures was 6.3%. While the false positive rate was 0.2%, the false-negative rate was 0.06%. In 11% of clinically significant isolates grown in blood culture bottles, growth was observed only in the anaerobic bottle. Most of the growth of Acinetobacter spp, Pseudomonas sp and yeast were detected in the aerobic bottle compared to the anaerobic bottle (P<0.05). Mean positive signal times were 18.5 and 20.9 hours for aerobic and anaerobic bottles, respectively. It has been concluded that the combined use of aerobic and anaerobic blood culture bottles and the volume of blood drawn are invaluable in the rapid detection of bloodstream infections.