The files here summarise the experimental data of a research work. The goal of this experiment was to link the hydrodynamic properties and age characteristics of transport (traced by a conservative tracer bromide) to reactive properties of Nitrate consumption by the native microbial communities inhabiting in a deciduous forest soil near Lausanne, Switzerland. To this aim, the native soil has been transported, sieved and packed to homogeneous soil columns (lysimeter) with porosity of 0.4. After several weeks of carbon amendment to boost the microbial communities, a pulsed of multi-tracers (named SPIKE) was applied on the 10th of June 2021. The data summarizes here the experimental conditions starting from the 25th of May 2021 to the 12th of August 2021.