The twenty bacterial replicons of the National Institute for Biological Standards and Control (NIBSC) Gut Community Standards were used as reference. It includes the following strains: Species Culture collection number Accession numbers Status Gut-Mix-RR Coverage (x) Gut-HiLo-RR Coverage (x) Akkermansia muciniphila DSM 22959 NC_010655 Complete genome 33,33 0,94 Alistipes finegoldii DSM 17242 NC_018011 Complete genome 16,94 4,85 Anaerostipes hadrus DSM 3319 NZ_KB290627 Complete genome 24,04 6,89 Bacteroides thetaiotaomicron DSM 2079 NC_004663 Complete genome 5,99 17,19 Bacteroides uniformis DSM 6597 GCF_000154205 Scaffold 10,81 3,1 Bifidobacterium longum subsp. infantis DSM 20088 NC_011593 Complete genome 29,52 84,63 Bifidobacterium longum subsp. longum DSM 20219 GCF_900104835 Contig 40,19 115,1 Blautia wexlerae DSM 19850 GCF_000484655 Scaffold 11,68 0,34 Clostridium butyricum DSM 10702 GCF_000409755 Contig 11,21 32,09 Collinsella aerofaciens DSM 13712 GCF_902501475 Contig 44,03 12,61 Escherichia coli DSM 1103 CP009072 Complete genome 8,86 25,34 Eubacterium hallii DSM 3353 GCF_000173975 Contig 21,79 6,25 Faecalibacterium prausnitzii DSM 17677 NZ_CP048437 Complete genome 24,66 0,72 Lactobacillus gasseri DSM 20077 NC_008530 Complete genome 66 1,91 Parabacteroides distasonis DSM 20701 NC_009615 Complete genome 10,2 29,26 Prevotella copri DSM 18205 GCF_000157935 Scaffold 19,23 55,11 Prevotella melaninogenica DSM 7089 NC_014370,1 and NC_014371,1 2 Chromosomes 23,49 6,73 Roseburia hominis DSM 16839 NC_015977 Complete genome 18,31 5,24 Roseburia intestinalis DSM 14610 NZ_LR027880 Complete genome 12,04 0,34 Ruminococcus gauvreauii DSM 19829 GCF_000425525 Scaffold 14,04 0,41 The raw sequence data of the mock communities, with an even and staggered distribution of species, is available at: SRR11487941 - Gut-Mix-RR Illumina MiSeq sample SRR11487935 - Gut-Mix-HiLo Illumina MiSeq sample