ABSTRACT The purpose of this research is to develop and validate a precise method for UV-Vis spectrophotometric and Reverse-Phase High Performance Liquid Chromatography (RP-HPLC) for determination of Octenidine dihydrochloride in bulk and pharmaceutical preparation. According to the relevant experiment the maximum wavelength was found to be 285nm and it is used for further process of development of method and its validation. The developed methods used for quantitative estimation of Octenidine dihydrochloride in pharmaceutical preparation and bulk drug which shows the satisfactory results as per ICH guidelines, so these developed and validated methods are found very simple, sensitive and rapid according to the ICH guideline and can be successfully applied to estimate the ODCL in bulk and pharmaceutical dosage form. Keywords: - ODCL, UV-Vis spectrophotometry, RP-HPLC, Development, Validation.