Untargeted metabolomics on ten different regulatory strains in Saccharomyces cerevisiae, (BY4741). Samples were taken before and after the diauxic shift, to investigate regulatory consequences of gene deletions and their roles during the substantial metabolic reconfiguration that is the diauxic shift. The analysis of samples was performed on an Agilent UHPLC-qTOF-MS system which consisted of a 1290 II Infinity series UHPLC system with a 6550 UHD iFunnel accurate-mass qTOF spectrometer. Data-set used in: High-throughput metabolomics for the design and validation of a diauxic shift model