ABSTRACT Bovine mastitis is economically important disease. Improper use of Antimicrobial agent for treating the condition will result in bacterial resistance and further economic losses. In the present study, the antibiotic sensitivity pattern was assessed in clinical bovine mastitis samples. The study was done during the period of April 2008 to March 2013 in various divisions of YSR district in Andhra Pradesh. A total of 257 subclinical mastitis samples were subjected to antibiotic sensitivity test by using various locally available antibacterial agents. Standard disc diffusion method was used to classify the bacterium involved as susceptible; intermediately susceptible; or resistant. From the study, the highest sensitivity was observed with ciprofloxacin, followed by chloramphenicol, enrofloxacin, amikacin and ofloxacin. Keywords: Antimicrobial Susceptibility Test (AST), Bovine mastitis, Disc diffusion method