Aim: The aim of the study is to evaluate prevalence of uropathogen and antibiotic susceptibility pattern in urinary tract infection in a tertiary care hospital.Method: This is a retrospective cohort study conducted over a period of 6 months in general medicine department of tertiary care hospital, Kerala. A total of 170 patients satisfying the inclusion criteria were analyzed. Case records were retrospectively reviewed for demographic data, clinical presentations, investigations, management and outcome. Data analysis were conducted using SPSS and Microsoft Excel 2010.Results: The majority of the patients causing urinary infection were females (61.2%) when compared with men (38.8%). Out of 170 patients, the age group >70 years were more prone to urinary tract infection. Of the total study group, Gram negative bacteria (97.1%) was the most prevalent organism causing urinary tract infection, among which E. coli (64.7%) predominates. E coli shows high sensitivity to amikacin. The most common antibiotic prescribed was found to be cefaperazone/sulbactam (30.6%).Conclusion: According to the study, urinary tract infections are highly prevalent in female patients >70 years of age. Regular surveillance and monitoring are extremely important to give practitioners the most recent information on the most efficient empirical treatment for urinary tract infections because antibiotic resistance among bacterial pathogens is evolving over time and space. Based on knowledge of the pattern of antibiotic susceptibility, empirical antibiotic selection should be made in the treatment of urinary tract infections.