The presence of 17 species of Chrysotoxum Meigen, 1803 (Ditpera, Syrphidae) has been confirmed in the East Mediterranean: Chrysotoxum antennalis Vujić, Nedeljković & Hayat, 2017; C. bicinctum (Linnaeus, 1758); C. cautum (Harris, 1776); C. cisalpinum Rondani, 1845; C. clausseni Vujić, Nedeljković & Hayat, 2017; C. elegans Loew, 1841; C. fasciatum (Muller, 1774); C. fasciolatum (De Geer, 1776); C. festivum (Linnaeus, 1758); C. impressum Becker, 1921; C. intermedium Meigen, 1822; C. lessonae (Giglio-Tos, 1890); C. octomaculatum Curtis, 1837; C. parmense Rondani, 1845; C. persicum Vujić, Nedeljković & Hayat, 2017; C. vernale Loew, 1841; and C. verralli Collin, 1940. In order to increase our knowledge of Chrysotoxum in this area we carried out new faunistic expeditions in Serbia, Montenegro, Italy, Greece and Turkey. Three species were found to be new to science, C. aff. octomaculatum (Turkey), C. aff. vernale (Greece and Turkey) and C. aff. intermedium (Greece). Chrysotoxum aff. octomaculatum can be separated from C. octomaculatum by the presence of black pile in the posterior part of the mesonotum and the colour of the basal part of the femora. Chrysotoxum aff. octomaculatum is also similar to C. verae Violovitsh, 1973, from which it can be distinguished by the yellow lateral margins of terga 3 and 4 and the colour of the basal parts of the femora. Chrysotoxum aff. vernale clearly differs from the similar C. vernale by the color of the wing pigmentation and the katepisternum. Chrysotoxum aff. intermedium can be separated from the similar C. intermedium by the presence of additional narrow longitudinal vittae on the mesonotum, and the presence of an area of pollinosity on the posterior part of the mesonotum. An ML analysis based on mtDNA COI gene and based on nuclear ITS2 sequences revealed a clear separation of C. aff. octomaculatum and C. aff. vernale from the other similar species.