hiPSC 3D immunofluorescence images, test data set 2x2, 10Z

Example dataset of human induced pluripotent stem cells, imaged at 40x magnification with a Yokogawa CV7000. This is a small subset of a larger experiment intended as a test dataset for Fractal: https://github.com/fractal-analytics-platform/fractal 3 Channels were imaged: - C01: DAPI, nuclear stain - C02: nanog, antibody staining with Bio-Techne AG, AF1997-SP, Lot KKJ0617121 for the stemness marker nanog - C03: Lamin B1, antibody staining with Abcam, ab16048, Lot GR3244890-2 for the nuclear envelope marker Lamin B1 This dataset contains 10 Z levels for 4 field of views for those 3 channels, as well as (manually adjusted) metadata files from the Yokogawa CV7000. The data was acquired in the Pelkmans lab in August 2020. The images have been converted from TIFF into PNG (lossless).

Related Organizations

Friedrich Miescher Institute
Switzerland

Keywords

image analysis, high content imaging, immunofluorescence

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