Aligned DNA sequence matrix for phylogenetic analyses of the article "Six new species of Pristimantis (Anura: Strabomantidae) from Llanganates National Park and Sangay National Park in Amazonian cloud forests of Ecuador". The matrix is in NEXUS format and contains the DNA matrix of sequences of 4 concatenated genes. Genes are arranged as follows: charset 16S = 1 -1386; charset RAG1 codon position 1 = 1387 -1966 \ 3; charset RAG1 codon position 2 = 1388 -1967 \ 3; charset RAG1 codon position 3 = 1389 -1968 \ 3; charset 12S = 1969 -2992; charset nonCoding = 2993 - 3068 4026 - 4155; charset ND1 codon position 1 = 3069 -4023 \ 3; charset ND1 codon position 2 = 3070 -4024 \ 3; charset ND1 codon position 3 = 3071 -4025 \ 3;