Aims & Objectives: The aim of the study was to examine the biochemical, antimicrobial, antioxidant and palynological studies of different honey samples collected from different locations of Dakshina Kannada, Karnataka, India. Materials & Methods: Ten types of honey samples were collected from ten locations of Dakshina Kannada. Physical properties and biochemical constituents were determined by following standard protocols. Antimicrobial activity test was done against Klebsiella pneumoniae and Streptococcus mutans by well diffusion method. Results: The color of honey samples were differed from light to intense and pH varied from 3.48 to 6.18. The protein content was in the range of 0.13-0.62 mg/g. The honey quality varied from 4.2 to 15 Brix %. Total sugar content was in the range of 4.5- 9.8mg/g. The total phenolic content varied considerably with the highest value obtained for Processed society honey, Butterfly Park, Beluvai. The different shapes of pollen grains observed. Conclusion: The honey samples exhibited different chemical composition and pH. The local natural honeys were abundant in specific plant pollens due to wider biodiversity both cultivated and natural vegetations in the collected areas. So local honey samples can be utilized as an excellent dietary source of antioxidants as well as the antibacterial agents, and hence duly be active for both therapeutic and prophylactic application.