This study aimed to determine the presence of Mycoplasma haemofelis (Mhf), which is a member of the hemotropic mycoplasmas called infectious anemia of cats, known to be zoonotic, usually progresses with hemolytic anemia in cats, and can lead to the death of the cat if not treated, in the Kırıkkale province by the Polymerase Chain Reaction (PCR) method. The experimental units of the study consisted of 50 male and 50 female cats of different breeds, aged between 2-months and 10-years, presented to the Kırıkkale University, Faculty of Veterinary Medicine, Internal Medicine, Surgery and Obstetrics Clinics with different complaints during January-August 2021. Smears prepared from blood samples were examined cytologically by the Giemsa. In addition, total blood count was done and the increased blood samples were analysed by PCR for the 16S ribosomal RNA gene. According to the PCR analysis findings of the study, it was concluded that the incidence of Mycoplasma haemofelis in Kırıkkale is 13 %.