A video to demonstrate the unique gait of a Varroa mite, captured as a 2DFT image. Here, the video first shows a bee emerging from her cell, followed by a Varroa mite walking and resting on brood-comb (panel ‘a’). These two concatenated videos run synchronously in time with the accelerometer recording taken from this footage (panel ‘b’). The accelerometer data is transformed into a 2DFT that updates with time, showcasing the frequency (Hz) and spectral repetition (Hz) of the vibrations produced by the bee, the mite and the background as they occur. The accelerometer recording has here been cropped (200 – 4000 Hz) and high-pass filtered (0 – 500 Hz) to best visualise the data of interest. The bottom panel ‘c’ showcases DF space, based on the PCA/DFA results of the 2DFT TDB, which are detailed later in section 3.5.4. The centroid of each of the three TDB DF clusters can be seen (bee = blue, mite = black, background = cyan). The red circle with black ‘tail’ represents the journey taken by the video-related accelerometer data in DF space, in relation to the PCA/DFA TDB results. This panel also runs synchronously in time with both panels ‘a’ and ‘b’. When the bee produces vibrations, the red circle remains in DF space near to the bee centroid, when the mite walks, the red circle moves to the vicinity of the mite centroid, and when the mite is stationary the red circle is found near the background centroid.