ABSTRACT The aim of the present work was to develop and validate a simple and efficient method for the analysis of Ambrisentan in pharmaceutical dosage forms by reverse phase high pressure liquid chromatography. A stainless steel column 150 mm long, 4.6 mm internal diameter filled with octasilyl silica chemically bonded with silica gel particles of 5 mm diameter was used for elution. The retention time of Ambrisentan was 4.451 min. The method showed a good linearity in the concentration range of 12.5-250 µg/mL with a correlation coefficient of 1.000. The validation characteristics included specificity, linearity, limit of detection, limit of quantification, precision, robustness and stability. Validation acceptance criteria were met in all cases. The method could be successfully used for the analysis of Ambrisentan in pharmaceutical dosage forms. Keywords: Ambrisentan, Accuracy, Precision, Linearity, Mobile Phase and Validation