The spreadsheet comprises measurements of methane production of biochemical methane potential (BMP) assays amended with graphene oxide and organic micropollutants. A total of six sheets are present. “DOE (VS)” contains the design of the experiment with the initial set-up value for the different conditions tested. “Stock solution” where the concentrations of the added contaminants are calculated. “Inoculum-substrate” stores the characterization of the inoculum and the substrate used (i.e., microcrystalline cellulose). “Final_Character” contains the characterization measurements carried out at the end of the experiment. “Data” envelops the periodic (mostly daily) measurements used to calculate methane production via a manometric procedure. “Calculation” has the final calculation reporting the specific methane production (SMP) for the different conditions.