Spectral flow cytometric tracking of mitochondria transfer in adipose tissue by age and diet

Unmixed fcs files for the stromal vascular fraction of epididymal (eWAT), inguinal (iWAT), and brown adipose tissue (BAT) in mtD2F/+AdipoqCre+/- and different ages or diets. For a breakdown of samples and code, please see: https://github.com/ncborcherding/mtD2. The full github repository at time of manuscript acceptance is also in the Zenodo repository under mtD2_GitHubRepo.zip. Antibody Panel: Laser Channel Antigen Fluorophore Final Dilution Factor Vendor Clone Catalog Number Violet V1 SiglecF BV 421 1:400 BD Biosciences E50-2440 562681 V2 CD44 Super Bright 436 1:300 eBioscience IM7 62-044-180 V3 CD11b Pacific Blue 1:300 BioLegend M1/70 101224 V5 IL-33R⍺-biotin n/a 1:300 BioLegend DIH9 145308 V5 Streptavidin (2°) BV 480 1:300 BD Horizon n/a 564876 V7 MHC-II (I-A/I-E) BV 510 1:300 BioLegend M5/114.15.2 107636 V8 Ly6C BV 570 1:400 BioLegend HK1.4 128030 V10 KLRG1 BV 605 1:300 BioLegend 2F1/KLRG1 138419 V11 F4/80 BV 650 1:300 BioLegend BM8 123149 V13 Ly6G BV 711 1:300 BioLegend 1A8 127643 V14 CD3 BV 750 1:300 BioLegend 17A2 100249 V15 CD206 BV 785 1:200 BioLegend C068C2 141729 Blue B1 mtDendra2 n/a n/a n/a n/a n/a B2 TCRb AF 488 1:300 BioLegend H57-597 109215 B3 CD8 Spark Blue 550 1:300 BioLegend 53-6.7 100780 B8 CD45 PerCP 1:200 BioLegend 30-F11 103130 B9 CD71 PerCP-Cy5.5 1:200 BioLegend RI7217 113816 B10 NKp46 PerCP-eFluor710 1:200 eBioscience 29A1.4 46-3351-82 Green YG1 TCRgd PE 1:300 BioLegend GL3 118108 YG3 CD64 PE/Dazzle 594 1:300 BioLegend X54-5/7.1 139320 YG4 CD4 PE/Fire 640 1:300 BioLegend GK1.5 100482 YG5 CD62L PE-Cyanine5 1:300 BioLegend MEL-14 104410 YG7 CD11c PE-Cyanine5.5 1:300 eBioscience N418 35-0114-82 YG9 CD25 PE/Cyanine7 1:200 BioLegend PC61 102016 Red R1 CD9 APC 1:300 BioLegend MZ3 124812 R2 CD127 AF 647 1:200 BioLegend A7R34 135020 R3 CD19 Spark NIR 550 1:300 BioLegend 6D5 115568 R4 CD90.2 AF 700 1:300 BioLegend 30-H12 105320 R5 CD5 BUV 737 1:300 BD Horizon 53-7-3 612809 R6 Viability dye Zombie NIR 1:1,000 BioLegend n/a 423106 R7 NK1.1 APC/Fire 750 1:300 BioLegend PK136 108752 R8 B220 APC/Fire 810 1:300 BioLegend RA3-6B2 103278

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University of Mary
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