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Abstract Smaragdiniseta musae is introduced as a leaf-based novel saprobic species from Musa. Multi-gene phylogenetic analyses of internal transcribed spacer (ITS), RNA polymerase II second largest subunit (rpb2), and β-tubulin (tub2) data support the taxonomic placement of the new collection in Smaragdiniseta (Stachybotryaceae, Hypocreales). The novel species is characterized by cup-shaped sporodochia covered by numerous peripheral setae and simple hyaline guttulate conidia produced by the ultimate branches (phialides) of conidiophores. This is the first report of Smaragdiniseta from Thailand and on Musaceae. In addition, we report Albifimbria verrucaria for the first time from Thailand based on morpho-molecular evidence. Keywords: 1 new species, banana, fungi, host, hyaline conidia, Musaceae, myrothecium-like, saprobes
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