This study aims to determine the antibacterial effect of the Ethanol Extract of Cocor bebek leaves (Kalanchoe pinnata) on the growth of Staphylococcus aureus and Salmonella typhi. The study is a true experimental, using a posttest only control group design with the independent variable being the extract of cocor bebek leaves (Kalanchoe pinnata). This study found that extracts of cocor bebek leaves (Kalanchoe pinnata) with a concentration of 7.5% and 15% had no inhibitory power, while at concentrations of 30% and 60% had weak inhibitory power as the minimum inhibitory power, and a concentration of 100% had inhibitory power, and strong on the growth of Staphylococcus aureus bacteria. Meanwhile for the growth of Salmonella typhi, a concentration of 7.5% from 3 repetitions had an average inhibition zone of 1.2 mm. for a concentration of 15% has an average inhibition zone diameter of 3 repetitions of 2.2 mm. concentration of 30% from 3 rolls has an average inhibition zone of 12.6 mm. for a concentration of 60% has an average inhibition zone of 3 repetitions of 19.8mm. There is antibacterial activity of ethanolic extract of cocor bebek leaf (Kalanchoe pinnata) against the growth of Staphylococcus aureus and Salmonella typhi.