Pre-eclampsia (PE) is a syndrome that affects multiple organ systems and is the most severe hypertensive disorder in pregnancy. It frequently leads to preterm delivery, maternal and fetal morbidity and mortality, and life-long complications (1). We currently lack efficient screening tools (2,3) and early therapies (4,5) to address PE. To study the initial stages of early onset PE and identify candidate markers and pathways, we performed spatio-temporal multi-omics profiling of human PE placentae and healthy controls and validated targets in early gestation in a longitudinal clinical cohort. We used a single-nuclei RNA-seq approach, spatial proteo-, and transcriptomics, and mechanistic in vitro signaling analyses. This repository contains the single nuclei processed data for decidua and placenta, and Visium (10x spatial transcriptomics) data used in the study. It contains the anndata objects (H5AD) for snRNA-seq with required metadata and NMF-based spot deconvolution data for visium (RDS). The raw Cellranger feature-barcode matrices (H5 files) per donor sample are also included. References 1 Williams, D. Long-term complications of preeclampsia. Semin Nephrol 31, 111-122, doi:10.1016/j.semnephrol.2010.10.010 (2011). 2 Brown, M. A. et al. Hypertensive Disorders of Pregnancy: ISSHP Classification, Diagnosis, and Management Recommendations for International Practice. Hypertension 72, 24-43, doi:10.1161/HYPERTENSIONAHA.117.10803 (2018). 3 Zeisler, H. et al. Predictive Value of the sFlt-1:PlGF Ratio in Women with Suspected Preeclampsia. N Engl J Med 374, 13-22, doi:10.1056/NEJMoa1414838 (2016). 4 Hauth, J. C. et al. Pregnancy outcomes in healthy nulliparas who developed hypertension. Calcium for Preeclampsia Prevention Study Group. Obstet Gynecol 95, 24-28, doi:10.1016/s0029-7844(99)00462-7 (2000). 5 Phipps, E. A., Thadhani, R., Benzing, T. & Karumanchi, S. A. Pre-eclampsia: pathogenesis, novel diagnostics and therapies. Nat Rev Nephrol 15, 275-289, doi:10.1038/s41581-019-0119-6 (2019).