You can find here the Direct RNA In Situ Sequencing (HybISS-based) maps generated using the Hight Sensitivity kit from CARTANA AB. They include half a mouse brain coronal section, targeting 50 genes. Genes were targeted in a sequential manner. Both reads, DAPI staining and segmented cells are included. The analysis of the same cells, but using 10X magnification are also provided in an anndata object.