### The code is used in the linux system. #Python environment and Cutadapt (version 2.10 or latest) needs to be installed before data processing. Installation on a Debian-based Linux distribution (https://cutadapt.readthedocs.io/en/stable/installation.html) ################ If there are hunderds and more cells are in scm6A-seq data, tso-mix separation is needed. sh ./scm6A-tsomix-separation.sh ${dir}/Test.fastq.gz Expcted output file: *index1.fastq *index2.fastq *index3.fastq *N4.fastq ############## After tso-mix separation,cellular barcodes is needed for single cell reads isolation. Or if less than 20 cells in one library, single cell reads isolation is performed directlty. ### Test 10 thousand reads for single cell isolation with 6 cores, cost 1m24.507s sh ./scm6A-SCbarcodes-isolation.sh Expected output files: *_${barcode}.fastq.gz