The distribution of secondary metabolites and pharmacologically dynamic complexes from marine microalgae has increased in the previous decade. The antibacterial metabolites lessons in the current search were taught by Chaetoceroscalcitrans. Methods :Chaetoceroscalcitrans(green algae) was chosen as the subject of the existing secondary metabolites study. The properties of pH, temperature, and salinity were tested to see if they aided in the growth of microalgae. Dissimilar solvent extracts of Chaetoceroscalcitrans were tested for antibacterial activity against Vibrio cholerae ,Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Salmonella sp.,Proteus sp., Streptococcus pyrogens, Staphylococcus aureus, Bacillus megaterium, and Bacillus subtilis. Result : The uppermost cell growth was detected during the 9th day of incubation when the medium was adjusted with pH of 9.0 in 30 ppt of salinity at 25C. Butanol + Isopropanol(1:1) crude extract of Chaetoceroscalcitrans had the largest zone of inhibition (13.4 mm) against Salmonella of all the solvents tested. . For the crude extract of Chaetoceroscalcitrans, GC-MS analysis revealed the presence of unique chemical compounds such as 3, 3, 5-Trimethylheptane (M.W. 142.2) and n-Hexadecane (M.W.226.2), respectively. Conclusion:These results show that the Butanol + Isopropanol (1:1) extract of Chaetoceroscalcitranshas significant antimicrobial activity and thus has great potential as a solvent for extracting bioactive compounds from natural sources for biomedical and pharmaceutical applications.