This glossary was created by the consortium partners of the European project HealthyCloud with the aim of establishing agreed definitions of commonly used terms to harmonise the work of the project. The aim is that the glossary can also be used by other projects working in similar areas. The glossary was created through a series of glossary working group calls, during which each definition was discussed and agreed upon. This is a living document that will be updated regularly with the addition of new terms and modifications, if needed, of the terms and definitions currently there. This is the second version of the HealthyCloud glossary, in which new terms have been added based on a need perceived in the glossary working group. Some existing terms have been modified to align with the recently published Proposal for a Regulation on the European Health Data Space (EHDS). In addition, sections have been created for terms relating to specific topics (e.g., artificial intelligence, profiles as defined in HealthyCloud).