Overview Data used for the manuscript: Nest shape influences colony organization in ants: spatial distribution and connectedness of colony members differs from that predicted by random movement and is affected by available space Purpose of the study Investigating how nest shape influences how Temnothorax rugatulus colonies spatially organize in their nests. This includes physical location of colony members and their distances from the entrance, mobile colony member distance to the brood center, worker distance to the physical center of the nest, and comparing worker distributions with those predicted by a random walk model. Structure of the data EMPIRICAL DATA WORKERS: FullDataCoordWorkers.csv, FullDataCoordWorkersRD2.csv Raw experimental data with worker x and y position in nests Colony: Unique experimental colony identifiers Nest: The nest shape treatment (Tube / Circle) Day: The experimental day that the observation was collected on ScaledX: X-axis coordinate, scaled from original (px) to (cm) in the software Fiji (Schindelin et al., 2012) ScaledY: Y-axis coordinate, scaled from original (px) to (cm) in the software Fiji ColorID: The unique color marking assigned to an individual worker's head, thorax, abdomen1, abdomen2 (i.e., Yellow, White, Green, Green = Y,W,G,G) Density: The density treatment (High / Low) BROOD / QUEENS: FullDataCoordBrood.csv, FullDataCoordBroodRD2.csv; FullDataCoordQueen.csv, FullDataCoordQueenRD2.csv Raw experimental data with brood (OR) queen x and y position in nests Colony: Unique experimental colony identifiers Nest: The nest shape treatment (Tube / Circle) Day: The experimental day that the observation was collected on ScaledX: X-axis coordinate, scaled from original (px) to (cm) in the software Fiji (Schindelin et al., 2012) ScaledY: Y-axis coordinate, scaled from original (px) to (cm) in the software Fiji Density: The density treatment (High / Low) ALATES: FullDataCoordAlate.csv Raw experimental data with alate (winged reproductive individuals) x and y position in nests Colony: Unique experimental colony identifiers Nest: The nest shape treatment (Tube / Circle) Day: The experimental day that the observation was collected on ScaledX: X-axis coordinate, scaled from original (px) to (cm) in the software Fiji (Schindelin et al., 2012) ScaledY: Y-axis coordinate, scaled from original (px) to (cm) in the software Fiji SexID: The unique sex assignment and number given to an individual alate: Sex, SexNumber, TotalNumber (i.e., the first male alate observation that came after three queen alates making it the fourth total observation = M,1,4) NETLOGO SIMULATIONS: ArchitectureMoveModelFull.csv Raw netlogo simulation data with agent x and y positions in nests RunNumber: The simulation number - 1 to 4000 - there are 1000 simulations for each combination of nest shape and size NestSize: The size of the nest area that agents were allowed to move throughout (Small / Large) Nest: The nest shape treatment (Tube / Circle) TimeStep: The duration of each simulation (should be 50000) xcor: a list of every agent x coordinate position at the end of the simulation ycor: a list of every agent y coordinate position at the end of the simulation REFERENCE DATA NEST BINS: Empirical BinsNullFull.csv Null data sheet with eight bins for tube and circle nests in every colony Colony: Unique experimental colony identifiers Nest: The nest shape treatment (Tube / Circle) Bin: Nest section identifier (1-8) BinCoordFull.csv Reference binning coordinates to group empirical coordinates into nest sections Colony: Unique experimental colony identifiers Nest: The nest shape treatment (Tube / Circle) CoordID: The unique coordinate identifier within each colony and nest combination ScaledX: X-axis coordinate, scaled from original (px) to (cm) in the software Fiji (Schindelin et al., 2012) ScaledY: Y-axis coordinate, scaled from original (px) to (cm) in the software Fiji NEST BINS: Netlogo Simulations BinsNullNetlogo.csv Null data sheet with eight bins for tube and circle nests in each simulation treatment Nest: The nest shape treatment (Tube / Circle) NestSize: The size treatment for simulations (Small / Large) Bin: Nest section identifier (1-8) BinCoordNetlogo.csv Reference binning coordinates to group Netlogo simulation coordinates into nest sections Nest: The nest shape treatment (Tube / Circle) NestSize: The size treatment for simulations (Small / Large) ScaledX: X-axis coordinate ScaledY: Y-axis coordinate CoordID: The unique coordinate identifier within each colony and nest combination CORNERS: Empirical CornerFull.csv Whether a nest section has a corner or not Colony: Unique experimental colony identifiers Nest: The nest shape treatment (Tube / Circle) Bin: Nest section identifier (1-8) Corner: Presence of a corner (Y / N) CORNERS: Empirical CornerFullSim.csv Nest: The nest shape treatment (Tube / Circle) Bin: Nest section identifier (1-8) Corner: Presence of a corner (Y / N) REFERENCE DATA DISTANCES IN THE NEST: Empirical DistBinsFull.csv Reference coordinates for the entrance of nest sections (Bin) front-to-back and shortest distance to the entrance from each nest section entrance Colony: Unique experimental colony identifiers Nest: The nest shape treatment (Tube / Circle) Distance: Reference shortest distance from a nest section to the entrance Bin: Nest section identifier (1-8) BinX: X-axis reference coodinate for a nest section entrance BinY: Y-axis reference coodinate for a nest section entrance Xmax: Max X-axis coordinate possible within the nest Ymax: Max Y-axis coordinate possible within the nest MaxDist: Max possible shortest distance from the nest entrance TubeRatio: Ratio of shortest distance to the nest entrance in circle nest / tube nest DISTANCES IN THE NEST: Netlogo Simulations DistBinsFullNetlogo.csv Reference coordinates for the entrance of nest sections (Bin) front-to-back and shortest distance to the entrance from each nest section entrance NestSize: The size treatment for simulations (Small / Large) Nest: The nest shape treatment (Tube / Circle) Distance: Reference shortest distance from a nest section to the entrance Bin: Nest section identifier (1-8) BinX: X-axis reference coodinate for a nest section entrance BinY: Y-axis reference coodinate for a nest section entrance Xmax: Max X-axis coordinate possible within the nest Ymax: Max Y-axis coordinate possible within the nest MaxDist: Max possible shortest distance from the nest entrance TubeRatio: Ratio of shortest distance to the nest entrance in circle nest / tube nest REFERENCE DATA WORKER SITE FIDELITY (SPATIAL FIDELITY & OCCURRENCE ZONE SIZES), ALSO RELATING SIZES TO DISTANCES IN THE NEST ColorRefFull.csv Reference of all possible unique color identifiers paint marked workers Colony: Unique experimental colony identifiers Head: Head color mark Thorax: Thorax color mark Abd1: Left side abdomen mark Abd2: Right side abdomen mark NestAreaFull.csv Reference for colony size (number of workers in the colony) and nest area Colony: Unique experimental colony identifiers Number.ants: Number of workers in the colony after painting Diameter: The diameter of the circle nest Area: The area of the nest ScalingCircleSFZ.csv Reference to scale the radius of circle nests to make coordinates representing fidelity zone bins Colony: Unique experimental colony identifiers Scaling: The scaling factor that is applied to the radius of each circle nest

Funding for this project was provided by: NSF grants DGE-1746060 to GC, IOS-3014230 and ABI-3019760 to AD